FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335234
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.042.4745061
GTATCAA30150.039.9663731
GTGGTAT4600.035.277411
TCAACGC36250.033.1849754
ATCAACG36500.033.1564873
TATCAAC37400.032.4751242
CAACGCA37150.032.3798185
AACGCAG37500.031.9856956
TGGTATC4900.031.6880972
ACGCAGA40750.029.3501057
CGCAGAG41900.028.6268168
CAGAGTA42100.027.67315710
AGAGTAC42150.027.56061611
GCAGAGT45100.026.519229
TAGTACC1501.0531949E-925.2769974
TCGTACG750.001822801123.35609662
CGCTTAT902.1310757E-422.99286724
AGTACGG42300.022.09101513
GAGTACG43050.021.70046412
TTAACGG3450.021.03921735
Produced by FastQC (version 0.11.5)