FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429018
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14660.3417106042170725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11310.2636253024348629No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11240.2619936692632943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9820.22889482492576071No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9570.22306756359873012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9520.22190211133332402No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTGGGCCGAACGTT9410.21933811634943057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8840.20605196052380087No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8460.1971945233067144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7980.1860061815588157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7890.1839083674810847No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA7820.18227673430951616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7730.18017892023178517No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC7520.17528402071707946No Hit
GCCCAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTC7290.16992294029621136No Hit
CCCCTAAGCTCCTGATCTATAAGTCGTCTACTTTAGAAAGTGGGGTCCCA6870.1601331412668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6800.15850150809523145No Hit
GTCTGCATCTGTGGGAGACAGAGTCTCCATCACTTGCCGGGCCAGTCAGA6790.15826841764215022No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6470.1508095231435511No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6200.14451608091035809No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6170.1438168095511144No Hit
CCCTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCACTTGCCG6110.14241826683262707No Hit
GTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAA6070.14148590502030217No Hit
CCTTGGTCCCTGGGCCGAACGTTCGAGAAAAATTATGATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGG6000.1398542718487336No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5860.13659100550559652No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5840.13612482459943406No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5490.12796665874159127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5470.1275004778354288No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATCATAATTTTTCTCGAACGTT5460.1272673873823476No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5350.12470339239845415No Hit
GTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGTCTA5290.12330484967996681No Hit
GTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGT5120.11934231197758602No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4990.11631213608753012No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4960.11561286472828647No Hit
ACTTATTACTGCCAACATTATCATAATTTTTCTCGAACGTTCGGCCCAGGGACCAAGGTGGACATCAAACGAA4940.11514668382212401No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG4810.11211650793206812No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG4780.11141723657282444No Hit
GTCCCTGGGCCGAACGTTCGAGAAAAATTATGATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG4680.10908633204201222No Hit
GAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGT4650.10838706068276856No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4630.1079208797766061No Hit
GGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGTCTACTTTAGAAAGTGGGGTCCCAT4590.10698851796428122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4560.10628924660503755No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4500.10489070388655021No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4450.1037252516211441No Hit
GCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT4420.10302598026190044No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC4370.10186052799649432No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT4330.10092816618416942No Hit
CTCCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCC4310.10046198527800698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)