FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429018
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14320.3337855288123109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10830.2524369606869642No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9930.23145881990965417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9270.21607485000629342No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9240.2153755786470498No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTGGGCCGAACGTT9190.21421012638164366No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9100.21211231230391264No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8780.20465341780531351No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8690.20255560372758252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8460.1971945233067144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8160.19020180971427772No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA7980.1860061815588157No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7610.17738183479481048No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7560.17621638252940436No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7330.17085530210853625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7110.16572731214074934No Hit
GCCCAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTC6850.15966696036063754No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC6680.15570442265825676No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6510.15174188495587598No Hit
CCCCTAAGCTCCTGATCTATAAGTCGTCTACTTTAGAAAGTGGGGTCCCA5960.13892191003640872No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5880.13705718641175896No Hit
GTCTGCATCTGTGGGAGACAGAGTCTCCATCACTTGCCGGGCCAGTCAGA5840.13612482459943406No Hit
CCTTGGTCCCTGGGCCGAACGTTCGAGAAAAATTATGATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGG5830.13589173414635283No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5770.13449319142786548No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC5470.1275004778354288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5440.12680120647618515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5410.12610193511694148No Hit
GTCCCTGGGCCGAACGTTCGAGAAAAATTATGATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG5290.12330484967996681No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5180.12074085469607335No Hit
GTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGTCTA5160.12027467378991091No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATCATAATTTTTCTCGAACGTT5030.11724449789985501No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA4990.11631213608753012No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4950.11537977427520524No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTGGGCCGAACGTTCGAGAAAAATT4900.11421432200979913No Hit
CCCTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCACTTGCCG4890.1139812315567179No Hit
GTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAA4830.11258268883823055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4800.11188341747898689No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4740.11048487476049956No Hit
ACTTATTACTGCCAACATTATCATAATTTTTCTCGAACGTTCGGCCCAGGGACCAAGGTGGACATCAAACGAA4730.11025178430741833No Hit
GTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGT4730.11025178430741833No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG4720.11001869385433713No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG4640.10815397022968733No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4580.10675542751119999No Hit
GAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGTCGT4460.10395834207422533No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4380.10209361844957553No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4370.10186052799649432No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4370.10186052799649432No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC4370.10186052799649432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)