FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555200
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA16920.1087962962962963No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT16120.10365226337448559No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC15950.10255915637860083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30900.041.0578771
GGTATCA13950.035.436491
TCAACGC37000.034.2855644
ATCAACG37050.034.1460043
CAACGCA37550.033.783385
AACGCAG39300.032.2790346
TATCAAC39550.032.0749932
ACGCAGA41750.030.2192297
CGCAGAG42800.029.558638
AGAGTAC42950.029.29349911
GTGGTAT5250.028.9722521
CAGAGTA44000.028.59444810
GTAGTTT8950.027.00000470
GCAGAGT47850.026.5104529
ATTAGAG5950.025.5612853
TTAACGG5400.024.99072635
TGGTATC6100.024.3660762
AGTACGG41050.023.74558813
TAACGGC5750.023.47559436
GAGTACG41650.023.40276312
Produced by FastQC (version 0.11.5)