FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555200
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT17340.11149691358024691No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA15950.10255915637860083No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC15600.10030864197530864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33950.041.747331
GGTATCA13300.038.727481
ATCAACG39100.035.7593653
TCAACGC39350.035.6073154
CAACGCA40350.034.7259675
AACGCAG40450.034.7254376
TATCAAC40850.034.5966532
ACGCAGA42850.032.699957
CGCAGAG44250.031.6643568
AGAGTAC45700.030.3605511
CAGAGTA46350.030.1552710
GCAGAGT49850.028.17569
TGGTATC6200.026.7517622
GTGGTAT6350.026.6751581
GTAGTTT10050.023.99004470
AGTACGG45500.022.99094213
GAGTACG45700.022.88664212
GTACCGA800.002866544821.6055161
GTACTTT15900.021.27915814
CGAACCG850.00411244220.3012585
Produced by FastQC (version 0.11.5)