FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245759
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTACAGCTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAAAA95680.7680458258780389No Hit
GCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTGATTGTTGGAGCTGTACATAACACT84100.6750904468681342No Hit
ATATTATACTCCTCCTCTAACGTTCGGCCAAGGGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCAT79350.6369610815575083No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT78410.6294154808434055No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76750.616090271071692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60350.48444362031500476No Hit
GTACATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTGTGGAGA58060.466061252617882No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54950.4410965523829248No Hit
GTATAATATTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC52930.42488153808240603No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52240.4193427460688624No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51350.4121985070948715No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATGTACAGCTCCAA50900.4085862514338648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47920.3846650917231985No Hit
GTCTCCACACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATGTA46450.37286505656391No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44600.3580146721797715No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTGTGGTCCCTTGGCCGAACGTT42060.33762549578208945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41680.3345751465572394No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41620.3340935124691052No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37570.3015832115200452No Hit
CCTCTAACGTTCGGCCAAGGGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG36200.2905858998409805No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC36170.29034508279691335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34900.28015049459807234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34400.27613687719695384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33810.271400808663634No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC33420.26827018709076156No Hit
AGGGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCA32780.26313275681732984No Hit
GTGTTATGTACAGCTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGCTG32630.2619286715969943No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTGCTGGTA32420.2602429522885245No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32000.25687151367158495No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31880.25590824549531654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31210.2505299981778177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30830.24747964895296765No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC30720.24659665312472154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29850.23961295884677536No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTGATTGTTGGAGCTGTA29650.2380075118863279No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC29080.23343198804905282No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28100.22556529794286054No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG28040.22508366385472633No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27520.22090950175756305No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25480.20453394276099954No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT25410.20397203632484293No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24960.20035978066383625No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT24480.19650670795876246No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT23630.18968355837686102No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA23480.18847947315652544No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATGTACAGCTCCAACAATCAGAACTA22650.18181686827066873No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGCTGCTCATT22330.17924815313395287No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22290.17892706374186337No Hit
GTGGTCCCTTGGCCGAACGTTAGAGGAGGAGTATAATATTGTTGACAGAA22050.1770005273893265No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22010.17667943799723704No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21870.17555562512492384No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21870.17555562512492384No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTGTGGTCC21790.17491344634074488No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21660.17386990581645406No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21290.17089982893962635No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT20860.16744811797466444No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTCCT20720.16632430510235124No Hit
CGTTAGAGGAGGAGTATAATATTGTTGACAGAAATAAACTGCCACATCTT20530.16479913048992623No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA20520.16471885814190385No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20410.1638358623136578No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20270.1627120494413446No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTCCTCCTCTAACGTT20050.16094605778485246No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19490.1564508062955997No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18990.15243718889448118No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18930.15195555480634698No Hit
CTACGGGGACGTCGTGATGACCCAGTCTCCACACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18790.1508317419340338No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACGTCGTGATGA18740.15043038019392194No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18630.14954738436567586No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTG18580.149146022625564No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18510.14858411618940742No Hit
CCACACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATGTACAG18420.1478616650572061No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18320.1470589415769824No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18270.14665757983687053No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18180.1459351287046692No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17960.14416913704817708No Hit
ATTATACTCCTCCTCTAACGTTCGGCCAAGGGACCACAGTGGAAATCAAA17910.14376777530806523No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC17860.14336641356795335No Hit
GTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTGATTGTTGGAGCTG17610.1413596048673941No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17470.1402357919950809No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17440.1399949749510138No Hit
GAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGCTGC17380.1395133408628796No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17230.13830925564254404No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17100.1372657151182532No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17070.13702489807418608No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17030.1367038086820966No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG16830.1350983617216492No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT16800.1348575446775821No Hit
GCCAAGGGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC16790.13477727232955974No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16770.134616727633515No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16700.1340548211973584No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTCCTCCTC16570.13301128067306758No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16500.132449374236911No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16490.13236910188888862No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16450.13204801249679915No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16430.13188746780075442No Hit
GGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGTCTCCACACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16210.13012147614426225No Hit
TTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTT15870.12739221631150166No Hit
ACATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTGTGGAGA15060.12089015612168966No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG14990.12032824968553307No Hit
GGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14820.11896361976915279No Hit
GTACAGCTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGCTGCTCATTTA14770.11856225802904094No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14730.11824116863695146No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14640.11751871750475011No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14520.11655544932848166No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14450.11599354289232508No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14160.11366564479967634No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14000.11238128723131842No Hit
GCCTACGGGGACGTCGTGATGACCCAGTCTCCACACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA13930.11181938079516184No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13910.11165883609911709No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13900.11157856375109472No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13790.11069556792284864No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13740.11029420618273679No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13740.11029420618273679No Hit
CCTCCTCTAACGTTCGGCCAAGGGACCACAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC13590.10909012096240124No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG13540.10868875922228939No Hit
GCCACAGTTCGTTTGATTTCCACTGTGGTCCCTTGGCCGAACGTTAGAGG13330.1070030399138196No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13180.10579895469348406No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC13110.10523704825732746No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTATGTACAGCTCCAACAATCA12970.10411323538501427No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12960.1040329630369919No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12810.10282887781665635No Hit
GTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGT12590.1010628861601642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC150.00239475968.7136463
GGTATCA8400.047.4603771
GTGGTAT2500.042.6161461
GTATCAA21900.040.645091
CTAGCGC1800.040.1264528
TGGTATC2800.039.264942
TAGCGCT1950.037.0457529
ACTAGCG2000.036.11235427
TCGTATG1550.035.685740
CTTATAC2100.034.367861
TATACAC2200.034.3568233
TATCAAC27050.033.0231932
ATCAACG27150.032.7750173
TCAACGC27350.032.5353474
CAACGCA28100.031.6669655
CGCTCAT2400.031.55835732
AACGCAG28600.031.1133466
CGTATGC1800.030.75170741
TACACAT2800.030.6757345
TTATACA2500.030.2340052
Produced by FastQC (version 0.11.5)