FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317282
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.039.424571
TACGTCG459.2549046E-538.8514658
GGTATCA15300.037.667311
TATCAAC37050.035.3836822
TCAACGC38250.033.911544
ATCAACG38700.033.606363
CAACGCA39250.033.1354455
AACGCAG40200.032.4382136
TATATCG450.0046243930.6646925
CGACATA450.00462524930.6635289
ACGCAGA43050.030.2895837
CGCAGAG44550.029.2697328
AGAGTAC44750.029.13891811
CAGAGTA45700.028.60867310
GCAGAGT48150.027.2246289
TTATACG1057.492836E-726.36207435
GTGGTAT4850.026.3268871
AGTACGG35700.024.3504513
CGATTGT1951.5024725E-624.33713570
CGTATGC1451.9035724E-823.92142341
Produced by FastQC (version 0.11.5)