FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372579
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35700.036.1052321
GGTATCA16300.033.9504361
TATCAAC43400.029.378392
ATCAACG44700.028.7546353
ACGTATC605.547712E-428.6382688
TCAACGC45050.028.4549484
CAACGCA45100.028.4234035
AACGCAG45700.028.2758356
ACGCAGA49800.025.8770087
CGCAGAG50150.025.5602978
AGAGTAC51100.024.68159111
CAGAGTA51550.024.6661310
GCAGAGT54500.023.520169
GAGTACG42800.021.7597312
AGTACGG43000.021.65852213
GTGGTAT7850.021.4552481
TACGGGC12150.020.64783515
TGGTATC8000.020.6203042
TTAACGG2352.0008883E-1120.57299435
TAGCGCC850.004161474820.25953131
Produced by FastQC (version 0.11.5)