FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372579
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17680.12880861502325183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42850.038.5410421
GGTATCA18250.035.9335141
TATCAAC49450.033.24122
ATCAACG49450.032.9491963
TCAACGC49900.032.5738224
CAACGCA50200.032.5169645
AACGCAG51900.031.5840136
ACGCAGA55800.029.3765287
CGCAGAG55550.029.323538
AGAGTAC56400.028.46219811
CAGAGTA56550.028.25921210
GCAGAGT59950.027.0559549
GAGTACG48050.025.27221112
AGTACGG48100.025.24685913
TGGTATC8200.023.4359022
TACGGGG22150.022.4573915
GTGGTAT8950.022.2502121
GTACGGG54550.022.01012414
GTATAGG1959.913492E-1021.1288491
TACGGGC12200.019.96473315
Produced by FastQC (version 0.11.5)