FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079021
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43970.40749901994493154No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACGCGGGAATCCGGGGTCCCT41630.38581269502632476No Hit
GTTTTATACACCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA31470.2916532671745962No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29250.27107906148258465No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAACACT29240.2709863848803684No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27910.2586603967856047No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25590.2371594250714305No Hit
GTCCTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT24540.22742838183872233No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23930.22177510910352996No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.2189948110370419No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT23510.2178826918104467No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22450.20805897197552228No Hit
GTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21770.201756963024816No Hit
ATATTATAGGACTCCTCCAACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT21740.20147893321816723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21630.20045949059378826No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA20790.1926746560076217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20060.18590926404583413No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19710.18266558296826477No Hit
TTATAGGACTCCTCCAACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT19600.1816461403438858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18970.1758075144042609No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17970.16653985418263406No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17960.1664471775804178No Hit
GTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACCCCGGATTCCCGCGT17430.1615353176629556No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA17200.15940375581198143No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16710.15486260230338425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16660.15439921929230294No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16060.14883862315932683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15900.14735579752386654No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15770.14615100169505507No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACGCGGGAA15440.1430926738219182No Hit
GTTTATTACTGTCAGCAATATTATAGGACTCCTCCAACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA15240.14123914177759284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14670.13595657545126555No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14580.13512248603131913No Hit
ATTCAATGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14480.13419572000915647No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14430.1337323369980751No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14380.13326895398699376No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14330.13280557097591242No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14080.1304886559205057No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13740.1273376514451526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13610.1261328556163411No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG13390.12409397036758321No Hit
GCTCATTTACTGGGCATCTACGCGGGAATCCGGGGTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATTT13300.1232598809476368No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13120.12159170210774396No Hit
GTCAGGGACCCCGGATTCCCGCGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA12950.1200161998700674No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACACCTCCAACAATAAGAACTA12950.1200161998700674No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12840.11899675724568845No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12820.11881140404125592No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12790.11853337423460711No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTAC12780.11844069763239085No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACTCCTCCAACTTT12670.11742125500801188No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12520.11603110597476786No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACACCTCCAA12450.11538236975925399No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACC12440.11528969315703773No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACT12370.11464095694152385No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12340.11436292713487504No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12140.11250939509054966No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12010.11130459926173819No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11970.11093389285287313No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11910.11037783323957549No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11860.10991445022849415No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11800.10935839061519655No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACCCCG11750.1088950076041152No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11680.10824627138860134No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11610.10759753517308746No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11610.10759753517308746No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACTCCTC11540.10694879895757359No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11430.10592935633319461No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11180.10361244127778792No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC11040.10231496884676017No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11020.10212961564232763No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG11000.10194426243789509No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10980.10175890923346256No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT10920.10120284962016496No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10890.10092481981351614No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10880.10083214321129988No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTGGAGG10840.1004614368024348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT300.003214101563.7929270
GGTATCA8550.043.194821
GTGGTAT2300.042.0188521
GTATCAA20000.039.692811
ATTAGAG1100.037.5869263
TATCAAC22600.035.2496382
TTAACGG1201.8189894E-1234.55882335
TTTAACG1201.8189894E-1234.5475834
TGGTATC2800.034.486662
ATCAACG23900.033.445553
TCAACGC23800.033.425814
TAACGGC1253.6379788E-1233.19035736
CAACGCA24550.032.404665
TGTTAGA6700.032.37252870
AACGCAG24850.031.8748686
AGAGTAC26100.029.95938911
CGCAGAG26600.029.9073078
GCAGAGT27650.028.896149
CAGAGTA27300.028.89083110
GTATTAG1452.1827873E-1128.5645431
Produced by FastQC (version 0.11.5)