FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195782
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAATGCCAGTGCCCCAGGCCTGACAGTAATAGTCAG38490.1752906253899522No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG38380.17478966491209055No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28620.13034080796727543No Hit
GCCCCAGAGGTGGAGCCTGAGAAACGATGAGGGATCCCGTCCCCCTTGGT28250.12865575908719537No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26820.12214327287499396No Hit
CCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGGC26300.11977509607055709No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24290.1106211818841761No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22920.10438194684171745No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22580.10283352354650871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10050.038.096891
GTATCAA50450.036.0998461
GGTATCA24100.036.067511
TGGTATC11750.033.750782
TATCAAC58050.031.1281152
ATCAACG60000.030.1157683
CAACGCA60550.030.0700175
TCAACGC60500.029.9801924
AACGCAG62150.029.3513776
ACGCAGA68400.026.8169947
AGAGTAC68450.026.39503711
CGCAGAG69250.026.3888558
CAGAGTA69700.026.07007810
ACGTTAG1501.150686E-425.17957170
GCAGAGT77400.023.8329249
AGTACGG63650.023.50963613
TCCGCGG10000.023.29110170
GAGTACG64650.023.19932212
ATTCGCG1258.7421824E-519.32099530
GTACGGG78200.019.0472214
Produced by FastQC (version 0.11.5)