FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319810
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATCTGGCCTCCGTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT74620.5653844113925489No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68340.5178018048052372No Hit
ATACTATACTGCTGACAGTAGTAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC61630.46696115349936734No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA59590.45150438320667363No Hit
CCTCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA46910.3554299482501269No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41180.31201460816329624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40170.30436199149877635No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT39640.3003462619619491No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39370.29830051295262194No Hit
GTCTCCAGCCACTCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTAGCAA35460.2686750365582925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35280.26731120388540774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34490.2613254938210803No Hit
CCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC33650.2549609413476182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33140.25109674877444477No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTAGCAACAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT32400.24548988111925205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31520.2388222547184822No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTACTACTGTCAGCAGTATAGTATCTGG31130.23586728392723197No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTAGCAACAAGTTAGCCTGGTA30710.2326850076905009No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT30660.23230616528136624No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT30440.2306392586811738No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC30030.22753275092626968No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAAC28830.21844053310703815No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27490.20828755654222955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25970.19677074730453625No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT23310.17661633113857297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22940.17381289731097657No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21920.16608451216462977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21770.16494798493722582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21730.1646449110099181No Hit
GTCTCTGGTGCCTCCATCAGCGGAGGTGATTACTACTGGGCTTGGATCCG21690.16434183708261038No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21600.163659920746168No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20250.15343117569953252No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19950.1511581212447246No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19580.14835468741712823No Hit
CTGCTACTCTGGCTCCCAGGTACCACTGGAGAAATAGTGATGACGCAGTC19400.1469908547442435No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19340.14653624385328193No Hit
GCAGTATAGTATCTGGCCTCCGTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19250.14585432751683955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19030.1441874209166471No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18990.14388434698933938No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18890.14312666217107006No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA18660.1413839870890507No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCGGAGGTGATTACTACTGGG18570.14070207075260832No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18470.13994438593433903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18460.1398686174525121No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18340.13895939567058896No Hit
CTATACTGCTGACAGTAGTAAACTGCAAAATCTTCAGACTGCAGGCTGCT18260.13835324781597352No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18180.13774709996135806No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT17950.1360044248793387No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG17750.1344890552428001No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17590.13327675953356924No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17500.13259484319712686No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17430.13206446382433834No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17410.13191292686068448No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17380.1316856214152037No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC17190.13024602026049203No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16910.128124502769338No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16490.12494222653260698No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16420.12441184715981846No Hit
GTTTACTACTGTCAGCAGTATAGTATCTGGCCTCCGTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16340.123805699305203No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACTCTGTCTGTGTCTCCAGG16190.12266917207779907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15720.11910805343193337No Hit
GTGAGAGTGTTAGCAACAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCCTCTATGGTG15170.11494078693145225No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCACTGGAGAAATAGTGATGACGCAGTCTC15110.11448617604049069No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15100.11441040755866373No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15030.11388002818587524No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14990.11357695425856752No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14940.11319811184943288No Hit
CTCCCAGGCTCCTCCTCTATGGTGCATCCACCAGGGCCACTGGTATCCCA14850.1125161955129905No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14690.11130389980375964No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14440.1094096877580864No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCCTCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT14350.10872777142164403No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTAGCAACAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGG14320.10850046597616324No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA14260.10804585508520166No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14190.10751547571241314No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14140.1071366333032785No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14080.10668202241231692No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14010.10615164303952843No Hit
GTAGTAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13980.10592433759404762No Hit
CCTCCTCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT13930.10554549518491299No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13520.10243898743000886No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13500.10228745046635501No Hit
ATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAACTTGTTGCTAACACTCTCACTGGC13400.10152976564808572No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13250.10039323842068178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.046.39321
GGTATCA10150.046.2017251
GTATCAA24200.040.3233261
TATCAAC27650.035.2893522
ATCAACG29850.032.688463
AGACGAA800.003461737432.5314270
TCAACGC30100.032.416964
CAACGCA30600.031.8884855
AACGCAG31400.031.1858466
CTCGAGT10050.030.42739970
GTAGTTT1354.4838136E-528.9168270
CGCAGAG34150.028.571418
AGAGTAC33850.028.41721311
GCAGAGT35000.027.680529
TAGGTAC756.172475E-527.59455922
ACGCAGA36200.026.9534177
TTTCGGC24700.025.69533722
TTCGGCG24850.025.54023623
TCACTGG21400.025.53868570
TAATAGC1659.822543E-1125.40273761
Produced by FastQC (version 0.11.5)