FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234971
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14470.11716874323364679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35800.036.835771
GGTATCA16950.035.7517241
TCAACGC41550.031.6918954
ATCAACG41850.031.3952733
CAACGCA42200.031.2037495
AACGCAG43050.030.666276
TATCAAC43000.030.5742112
ACGCAGA45400.029.0031937
CGCAGAG46700.028.1222048
AGAGTAC47500.027.42921311
GTGGTAT7500.027.0859931
CAGAGTA49100.026.60540210
GCAGAGT52050.025.4948169
TGGTATC7750.025.312412
GAGTACG38200.024.1178812
AGTACGG38350.024.11709413
TTAACGG2800.022.18932335
GTATTAG4100.021.8345131
GAGTACT17800.021.63045912
ACGGGTA2251.0913936E-1121.3970516
Produced by FastQC (version 0.11.5)