FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526442
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5600.10637449139696302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.039.1260831
GTATCAA15500.038.4983371
GCGTGTA754.0008672E-836.81323632
ATACGTG501.832394E-434.5880438
TATCAAC17800.033.514282
TCAACGC17950.033.231064
ATCAACG18150.032.864883
CAACGCA18600.032.069765
AACGCAG19300.030.9066056
GTGGTAT2600.029.1861551
ACGCAGA20950.028.4724357
AGAGTAC21200.027.97403511
CGCAGAG21350.027.7774968
TATACGT500.007667605827.64672537
GCAGAGT22600.026.5462599
GTAAATC658.6807285E-426.52278716
CAGAGTA22450.026.4164610
ATTAGAG809.59062E-525.8597183
GCACTAT2651.2751116E-924.84826370
AGTACTT8850.024.15521813
Produced by FastQC (version 0.11.5)