FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571523
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGT6580.11513097460644629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA305.8641797E-446.6870859
GTATCAA18900.041.6454961
GGTATCA6900.038.0241471
TCAACGC22150.035.5256544
CAACGCA22250.035.2108765
GACGTAT400.0024226834.9873658
ATCAACG22750.034.5887153
GTATTAT400.00258717534.5219231
ATAATGC400.0025916234.50984213
CTTATCG400.0025916234.5098428
AACGCAG22950.034.1369066
ACGCAGA24050.032.5726977
GTGGTAT2000.031.0697331
GCAGAGT25600.030.7353279
AGAGTAC25150.030.59918211
CGCAGAG25750.030.5562888
TATCAAC25850.030.4407462
CAGAGTA25750.029.88619410
TACCCGT605.2972E-428.8617340
CGTATAG500.0071701328.02966960
Produced by FastQC (version 0.11.5)