FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700493
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGATGATCACTACTACTATCCCACAC38400.5481853494610225No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGATGATCACTA36300.5182064631623728No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32530.46438722442622554No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG31880.45510804533378635No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC29410.41984716478251743No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28550.4075700970602133No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT27380.3908675746938228No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24630.35160950930273394No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT23710.3384759019718969No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23120.3300532624879906No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23050.3290539662780356No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22990.32819742666950275No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22700.32405748522826067No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC22280.3180617079685308No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21720.3100673382888908No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21570.3079259892675587No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21310.30421431763058304No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20650.2947923819367217No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC19490.2782326161717533No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19060.2720940823106012No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18210.25995977118971925No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT18120.25867496177691995No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC18020.25724739576269856No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC17750.25339296752430074No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17440.24896751288021438No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17160.24497032804039442No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC16860.24068762999773016No Hit
GGATAGTAGTAGTGATCATCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16750.23911730738208659No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16710.23854628097639805No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16580.2366904451579102No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16580.2366904451579102No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT16390.23397806973088953No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT16350.23340704332520096No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16250.23197947731097954No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16190.23112293770244668No Hit
GTAGTAGTGATCATCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA16070.229409858485381No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG16070.229409858485381No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC15880.22669748305836035No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA15810.2256981868484053No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15630.22312856802280678No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG15480.2209872190014747No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG15410.21998792279151969No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15140.2161334945531219No Hit
TAGTAGTAGTGATCATCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC14980.21384938893036762No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14790.21113701350334693No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA14760.21070874369908052No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGATGATCACTAC14690.2097094474891255No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14200.20271437401944062No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14100.2012868080052192No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14010.2000019985924199No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG13890.1982889193753542No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG13850.19771789296966566No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13750.19629032695544424No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13730.19600481375259995No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13640.1947200043398007No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13410.19143660250709144No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13400.19129384590566928No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG13310.19000903649287001No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG13150.18772493087011577No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12730.18172915361038583No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12660.18072985740043085No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12290.1754478631478116No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11790.16831003307670456No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATCAGGTGTTCGGCG11690.16688246706248314No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11590.16545490104826174No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11480.16388457843261817No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT11360.1621714992155525No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG10970.15660399176008896No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10940.15617572195582252No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10850.15489091254302326No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10770.15374885973164612No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCC10600.15132199750746972No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC10600.15132199750746972No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10460.14932340508755976No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA10390.14832410887760478No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10340.14761032587049405No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGATGATCA10320.14732481266764977No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG10210.14575449005200622No Hit
GTGTGGGATAGTAGTAGTGATCATCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9970.14232833161787484No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC9920.14161454861076414No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9880.14104352220507557No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9810.14004422599512056No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9750.13918768638658774No Hit
GCTTGGTCCCTCCGCCGAACACCTGATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC9660.13790287697378845No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC9510.13576152795245633No Hit
AGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCC9470.13519050154676776No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9190.1311933167069478No Hit
CCTGATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCA9180.13105056010552568No Hit
GACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG8880.12676786206286145No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA8820.1259113224543286No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT8820.1259113224543286No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8760.12505478284579574No Hit
GCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT8710.12434099983868505No Hit
AAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC8680.12391273003441862No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC8620.12305619042588577No Hit
GACTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATCAGGTGTTCGG8530.1217713810130865No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8470.12091484140455365No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT8410.12005830179602081No Hit
GGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAG8370.11948727539033224No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8300.11848797918037725No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG8260.1179169527746887No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT8210.11720316976757798No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG8170.11663214336188941No Hit
CCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT8150.11634663015904513No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8090.11549009055051229No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8080.11534733394909015No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8050.11491906414482372No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8040.11477630754340158No Hit
CCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA8000.11420528113771301No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC7850.1120639321163809No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC7780.1110646359064259No Hit
CTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA7730.1103508528993152No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7730.1103508528993152No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGC7710.11006533969647092No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG7710.11006533969647092No Hit
CATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG7580.10820950387798309No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGC7520.10735296426945024No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC7460.10649642466091738No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7460.10649642466091738No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7450.10635366805949525No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG7270.10378404923389671No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC7220.103070266226786No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT7210.10292750962536386No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT7210.10292750962536386No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT7200.10278475302394172No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG7170.10235648321967529No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG7010.10007237759692103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1550.051.1025543
AGGGGGG1400.048.72630770
CGTATGC506.7162546E-848.5015341
TTATACA1450.047.5050162
GGTATCA6150.047.048271
TCGTATG451.8158171E-646.16209840
GTATCAA17350.042.685311
TATCTCG651.1228622E-842.52266736
ACCGATC1400.041.8333327
CCCACGA1500.041.32641619
CGATCAG1550.040.01615529
TATCAAC18750.039.125132
GACCGAT1600.038.75457826
CTTATAC1700.038.498531
AGTATCG459.985932E-538.35006734
TCAACGC19500.037.7942434
CCGATCA1550.037.79033728
ATCAACG19500.037.6176343
CAACGCA19950.037.1143655
ATGCCGT753.8307917E-836.9987244
Produced by FastQC (version 0.11.5)