FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830455
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18570.223612357081359No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16830.2026599875971606No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14940.17990137936432438No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13930.1677393717901632No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13850.1667760444575564No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13420.16159816004479471No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12900.15533653238285036No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12650.15232613446845403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12590.15160363896899892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12120.14594409088993385No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11680.14064579056059628No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11470.1381170563125034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11040.1329391718997417No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10730.12920627848589025No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10420.12547338507203884No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTAC10290.12390797815655273No Hit
GTGTATCTTGCATGCAATAATAAACCCCAACATCCCCAGCCTCCACCGTGCTGATTTTCAGTGTGAAATCAGTGC10280.12378756223997688No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10260.12354673040682518No Hit
CTATAAGGATCTCTGGCACAGAAATATACGGCCGTGTCCTCGAGTCTCAG10210.12294465082394591No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10210.12294465082394591No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10060.12113841207530812No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9260.11150513874923988No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9220.11102347508293647No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9160.11030097958348135No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9160.11030097958348135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9090.10945806816745036No Hit
CAAGATACACTGTTCACTTCCGGCACTGGGACCAAAGTGGATATCAAACG9050.10897640450114696No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9020.10861515675141942No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA8790.10584559067017478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8740.10524351108729552No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC8700.10476184742099211No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGAG8360.10066770625741311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG306.200195E-446.16479540
GTGGTAT1850.041.062411
GTATCAA13750.037.166571
TATCAAC15500.032.7416532
GGTATCA10400.031.54161
ATCAACG16400.030.9448593
TCAACGC16400.030.9448594
CGTATGC450.004531073430.79140741
CAACGCA16850.030.1184395
AACGCAG16900.030.029336
TGTGTCG850.006090313728.98131470
AACGTAC605.3974456E-428.7713323
TACTACG500.007708032627.618812
TGTTAGA3800.027.55131770
CGCAGAG18600.027.094218
CAGAGTA18750.027.06154810
AGAGTAC18500.027.05408511
GCAGAGT19750.025.6913419
CTAATAC1359.3514245E-925.5729733
CAACGTA700.001327784824.66113922
Produced by FastQC (version 0.11.5)