FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875136
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23550.040.447571
CTCGTAT952.1282176E-1036.41239
GGTATCA9800.033.6859441
ATCAACG29300.032.6216773
TCAACGC29800.032.0743334
TATCAAC29950.031.7988952
CAACGCA30500.031.4491275
AACGCAG30650.031.1830486
TCGTATG1151.6352715E-930.11410940
TGGTATC4700.029.2611392
GTGGTAT4950.028.482771
ACGCAGA33650.028.3964927
AGAGTAC33750.028.1086711
CGCAGAG35050.027.2622538
CAGAGTA34950.027.0452210
GCAGAGT37550.025.447199
TTGTACG700.001360518924.5584563
GAGTACG30550.024.4148212
AGTACGG31100.024.31460813
GTGTATA2057.6397555E-1121.79688613
Produced by FastQC (version 0.11.5)