FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019999
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25900.2539218175704094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18810.18441194550190737No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18390.180294294406171No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16900.1656864369474872No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAAATA16340.16019623548650538No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCCCCTGGCCGAACGTC16180.15862760649765342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16100.15784329200322747No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14870.14578445665142808No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14340.140588373125856No Hit
GATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14220.13941190138421702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13890.13617660409470989No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13550.1328432674933995No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13510.13245111024618653No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13350.13088248125733457No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGATATGATTTAAGCTGGTATCAGCAGAAA13290.13029424538651507No Hit
CTCCTGATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTT13090.12833345915045016No Hit
GTATTATTGTGTGAGAGAGATTCATGGGTATGATTACGTTTGGGAAAACTATCGTTATAGGAGCTACTACTTTGA12840.12588247635536898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12540.12294129700127158No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT12410.12166678594782937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12270.12029423558258391No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12150.11911776384094495No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11880.11647070242225728No Hit
GTAATAAGTTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG11650.1142157982507826No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11490.11264716926193065No Hit
CCATAGATCAGGAGAGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCT11300.11078442233766896No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11060.10843147885439104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10900.1068628498655391No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10350.1014706877163605No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10340.10137264840455727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.041.6587871
CTAGCGG350.001286153739.80550456
GTATCAA20250.039.237791
TATCAAC22900.034.690372
ATCAACG23900.033.2388843
CAACGCA24350.032.766465
TCAACGC24250.032.7591484
AACGCAG25050.031.8508326
GTAGACA9050.030.08145970
ACGCAGA27400.029.1176727
CGCAGAG27300.029.0978188
AGAGTAC26650.029.02993211
CAGAGTA27150.028.74973310
GCAGAGT28800.027.4623839
ATAATAC4700.025.7209323
TAATACA5050.024.6222464
CGTTATA4450.024.22992553
CTATCGT4600.023.40861749
AGTACGG27450.023.40269313
TCGTTAT4650.023.17977152
Produced by FastQC (version 0.11.5)