FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019999
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24570.24088258910057755No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCCCCTGGCCGAACGTC16960.16627467281830668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16830.16500016176486448No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16590.16264721828158654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15720.15411779815470408No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15660.1535295622838846No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAAATA14860.14568641733962484No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14210.1393138620724138No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13680.1341177785468417No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13610.133431503364219No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13590.13323542474061248No Hit
GATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA13560.13294130680520275No Hit
CTCCTGATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTT13350.13088248125733457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13260.13000012745110534No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13000.12745110534422094No Hit
GTATTATTGTGTGAGAGAGATTCATGGGTATGATTACGTTTGGGAAAACTATCGTTATAGGAGCTACTACTTTGA12700.12450992599012352No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12570.12323541493668132No Hit
GTAATAAGTTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG12340.12098051076520663No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11980.11745109554028976No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGATATGATTTAAGCTGGTATCAGCAGAAA11910.11676482035766701No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11620.11392168031537286No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT11490.11264716926193065No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11140.10921579334881701No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11100.10882363610160402No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11030.1081373609189813No Hit
GTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT10760.10549029950029364No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10580.10372559188783519No Hit
CCATAGATCAGGAGAGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCT10500.1029412773934092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19050.040.630621
GTGGTAT2800.039.4904751
TATCAAC20950.036.574542
CCGCGTA400.002602476234.4830139
ATCAACG22300.034.0274543
TCAACGC22800.033.1234634
CAACGCA23350.032.341675
AACGCAG23650.032.077226
AGAGTAC26450.029.07691211
TAGTACG605.435865E-428.7372534
CGCAGAG26500.028.6274058
ACGCAGA26650.028.4662767
ATAGTAC854.436033E-628.4047373
CAGAGTA27150.028.32306310
GCAGAGT28000.027.3401059
ATAATAC4200.027.1004373
AATAATA4400.025.8863742
TAATACA4400.025.8635274
GATTACG4050.024.74005532
ATCGTTA3950.024.64300251
Produced by FastQC (version 0.11.5)