FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984221
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23910.24293324365157826No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23500.23876751258101583No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT17070.1734366570109762No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA16510.16774687798776902No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCGGGGATACCAGTGGCCCTGGT16500.16764527479092603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14890.15128716009920537No Hit
GTAATAGACGGCCGTGTCCGCAGCACTCACAGAGCTCAGCTTCAGGGAGA14820.15057593772130445No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14100.1432605075486095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13940.14163485639912174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13910.1413300468085928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13120.13330339425799692No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12570.12771521843163272No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12110.12304147137685541No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT11850.12039978825893777No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG11740.11928215309366494No Hit
GTCTCTGGTGGCTCCATCAGTAGCTACTACTGGAACTGGATCCGGCAGCC11660.11846932751892106No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11570.11755489874733419No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCCGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAGTTCA11180.11359237407045776No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11090.11267794529887089No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10610.10780099185040758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10590.1075977854567216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10080.10241602241772936No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT9900.10058716487455562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.046.2069741
GTATCAA25200.040.907841
TATCAAC28450.036.1006852
TCAACGC28750.035.4860274
CAACGCA29000.035.061265
ATCAACG29200.035.055413
GTGGTAT2500.034.4775121
AACGCAG29650.034.2926336
TCCCGAT6150.033.29921370
AGAGTAC31900.032.1963811
CGCAGAG32650.031.2472658
ACGCAGA32800.030.9992857
CAGAGTA34000.030.31068610
GCAGAGT33850.030.2413589
AGCGTTG605.4537057E-428.72104312
AGTACGG30550.028.54241813
GAGTACG30900.028.33065812
TGGATTG6400.028.11986470
GTAGACG600.00789664327.48228669
TTCCCGA5400.026.71889169
Produced by FastQC (version 0.11.5)