FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011692
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA350.00782973250.94163570
GTATCAA18200.038.546241
GGTATCA8100.038.3985521
ATCAACG21450.032.7026633
CAACGCA21500.032.3051725
TCAACGC21950.031.6428784
AACGCAG22950.030.5652396
TATCAAC22750.030.530162
ACGCAGA24750.028.342317
GTATAAG3650.027.4575961
CGCAGAG25750.027.1074458
AGAGTAC25200.026.87633111
CAGAGTA25750.026.43646810
ATATGAC1752.3646862E-1023.695053
AATAGCG2103.6379788E-1223.33206462
GTACTAG902.1017711E-423.0391311
GCAGAGT30600.022.8119
GTGGTAT3800.022.7359851
AGTACGG20600.022.64545213
TAACGGC2450.022.60581836
Produced by FastQC (version 0.11.5)