FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011692
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.044.1780621
ATCAACG23550.038.3566473
GGTATCA8150.038.1312641
TCAACGC23750.037.7414444
CAACGCA23950.037.714175
TATCAAC23850.037.455312
AACGCAG25050.036.3333136
ACGCAGA26900.033.706397
CGCAGAG27950.032.316798
AGAGTAC28000.031.40185711
CAGAGTA28350.031.25124710
GCAGAGT31350.028.8105189
AGTACGG22900.028.16071713
GAGTACG23200.027.79519812
GTACGGG27000.023.76026214
TGGTATC3400.023.3319452
GTCGTAC750.001982816323.0199871
TTTAGCG902.1178916E-423.01315126
GTGGTAT3700.022.3978251
TTAACGG1756.044502E-921.73361235
Produced by FastQC (version 0.11.5)