FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109273
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31300.039.762511
GGTATCA13650.038.788021
TATCAAC39700.031.863142
TCAACGC39600.031.7699934
TCGTATG1207.4578566E-1131.74859840
ATCAACG39900.031.5311223
CAACGCA40350.031.0942865
AACGCAG40850.030.7978426
CGTATGC1551.8189894E-1229.07498241
ACGCAGA44150.028.5711337
GCGTAGA1055.621561E-428.57069670
CGCAGAG45200.027.831388
AGAGTAC45700.027.30124911
CAGAGTA45750.027.27141410
GCAGAGT48150.026.0548559
GAGTACG38200.025.193412
AGTACGG38250.025.07060613
GTGGTAT6250.023.1035561
TACGGGA13000.022.73773415
TGGTATC6350.022.1942962
Produced by FastQC (version 0.11.5)