FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043660
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28410.27221508920529675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20860.19987352202824674No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCCCAGGGCCGAAAGTC19270.18463867543069581No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19180.1837763256232873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18550.17773987697142749No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17480.16748749592779258No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16920.1621217637928061No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16550.15857654791790432No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA16490.1580016480462986No Hit
AGCCAGGGCAGTGTCCTCAGCTCCTGATCTATTTGGGTTCTTATCGGGCC16420.1573309315294253No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15460.14813253358373416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15180.14544966751624092No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15020.14391660119195906No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14970.143437517965621No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG14970.143437517965621No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14520.13912576892857825No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14110.135197286472606No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13930.13347258685778896No Hit
GGTCTAGTCAGAGCCTCCTGCATAGGAATGGATACAAGTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTGTC13530.1296399210470843No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13190.12638215510798537No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12810.12274112258781596No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12380.11862100684130847No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12200.1168963072264914No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12200.1168963072264914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12150.1164172240001533No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAACCCAAATAGATCAGGAGCTGAGGACACTGCCCTGGCTTCTGCAGGTA12120.11612977406435046No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11950.11450089109480098No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11940.11440507444953336No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11540.11057240863882874No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATACTTGTATCCATTCCTATGC11320.10846444244294118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCC11260.10788954257133548No Hit
GCATAGGAATGGATACAAGTATTTGGATTGGTACCTGCAGAAGCCAGGGC10900.10444014334170132No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10690.10242799379108138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10610.10166146062894046No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10590.10146982733840522No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG10550.10108656075733477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17300.039.738191
GTGGTAT2400.038.8645481
GGTATCA7500.036.3887331
TATCAAC20100.034.5429572
TGGTATC3000.033.391522
TCAACGC20800.033.380454
CAACGCA20850.033.30045
ATCAACG20900.033.2207373
AACGCAG21700.032.3143776
GTTCTAA450.004592294330.7077921
GTAGTTT2459.094947E-1230.61791470
TTAACGG802.7041788E-630.26859535
TAACGGC703.777116E-529.65513436
AGAGTAC23550.029.47969611
CGAACTA953.1566742E-729.10135529
CAGAGTA24350.028.65301110
ACGCAGA24900.028.1601757
CGCAGAG25050.028.129448
TACTATA1057.5140633E-726.35253134
TACTGAT7900.025.32114470
Produced by FastQC (version 0.11.5)