FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043660
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27810.2664660904892398No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCCCAGGGCCGAAAGTC19630.18808807466033No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18640.17860222677883603No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18000.1724699614817086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17180.1646129965697641No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16760.16058869746852425No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15830.15167774945863594No Hit
AGCCAGGGCAGTGTCCTCAGCTCCTGATCTATTTGGGTTCTTATCGGGCC15520.14870743345533985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15410.14765345035739608No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.14564130080677615No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG14900.14276680144874768No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA14890.14267098480348006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14780.1416170017055363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14780.1416170017055363No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14540.1393174022191135No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14470.13864668570224017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14220.13625126957054978No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13480.12916083782074622No Hit
GGTCTAGTCAGAGCCTCCTGCATAGGAATGGATACAAGTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTGTC13340.1278194047869996No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC13240.12686123833432345No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12500.11977080658451987No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12190.11680049058122377No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12100.11593814077381522No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11970.11469252438533623No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11950.11450089109480098No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11780.11287200812525153No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAACCCAAATAGATCAGGAGCTGAGGACACTGCCCTGGCTTCTGCAGGTA11520.11038077534829349No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCC11090.10626065960178603No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT10960.10501504321330701No Hit
GTCCTCAGCTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCT10870.10415269340589847No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG10710.10261962708161662No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATACTTGTATCCATTCCTATGC10620.10175727727420808No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10610.10166146062894046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)