FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591330
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20190.12687500392753231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52750.039.79381
GTGGTAT8300.039.3870661
GGTATCA22000.037.853041
TGGTATC9100.035.5138782
ATCAACG62000.033.5128173
TATCAAC62450.033.417032
TCAACGC62550.033.321674
CAACGCA63950.032.5921865
AACGCAG64550.032.3424346
CGCAGAG69700.029.9527158
ACGCAGA69900.029.817897
CAGAGTA71950.028.82514810
AGAGTAC71600.028.72807311
GCAGAGT76400.027.415869
GAGTACG64950.024.21621512
AGTACGG65250.024.16130413
CGGGATA1902.7284841E-1123.50424217
AGTACTT22300.021.40908813
GTAGTTT7700.021.02024570
CGTACGC1004.4706877E-420.606233
Produced by FastQC (version 0.11.5)