FastQCFastQC Report
Wed 8 May 2024
EGAF00005760372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957522
Total Bases71.8 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA32060.33482259415449467No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28120.2936747145235305No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT26780.279680257999294No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTA26350.2751894995624121No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24830.25931519066924835No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA22730.23738357969842994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22120.23101296889262074No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21520.22474679432952976No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21410.22359799565962973No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA20550.214616478785866No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18410.19226712284417485No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG18380.1919538141160203No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18310.19122276041699304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17870.18662756573739298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17850.18641869325195662No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17540.18318116972769294No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17030.1778549213490656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16430.17158874678597463No Hit
ATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCA16350.17075325684422915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16280.1700222031452019No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16130.16845565950442914No Hit
GTCCAGAGACAACGCCAAGAATTCATTGTCTCTGCAAATGAACAGCCTGA15890.16594918967919275No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15670.16365159233939272No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15580.16271166615492907No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCACGTCATCCAATAAGTAC15470.16156286748502907No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT14380.15017931702874712No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14330.14965713581515622No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14240.14871720963069257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14120.14746397471807435No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13930.1454796861064289No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACTTATTGGATGACGTGGG13730.1433909612520652No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC13500.14098892766954702No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13340.13931794778605608No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAG13320.13910907530061972No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13210.13796027663071972No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13190.13775140414528333No Hit
CAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGA13000.13576711553363788No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12930.1350360618346106No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12840.13409613565014694No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC12630.1319029745530651No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC12490.13044086715501055No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12440.12991868594141961No Hit
GGGCTGGAGTGGGTGGCCAGCATAAAGGAAGATGGAAGTGAGAAATTCTA12220.1276210886016196No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12220.1276210886016196No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCAC12190.12730777987346506No Hit
GCATAAAGGAAGATGGAAGTGAGAAATTCTATGTGGACTCTGTGAAGGGC12180.12720334363074687No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12070.12605454496084686No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAA11930.12459243756279229No Hit
ATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCACCATCAGCAGCC11890.12417469259191957No Hit
GTCTCTGGACATGGTGAATCGGCCCTTCACAGAGTCCACATAGAATTTCT11770.12292145767930138No Hit
GTCCAGTGTGACGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCC11560.12072829658221954No Hit
CCTTTAGTACTTATTGGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGG11320.11822182675698314No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11230.1172819005725195No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT11190.11686415560164674No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC11080.11571535693174675No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTTGGAGGTGTCCA11050.1154020482035922No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11010.11498430323271945No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10980.1146709945045649No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA10930.11414881329097401No Hit
CTAGTAGCTCGCGCACAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCT10750.11226896092204669No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT10610.11080685352399214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10590.11059798103855577No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCTCAGACTTTTGGCCAGGG10580.11049354479583759No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10420.10882256491234665No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG10290.10746489375701028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10110.10558504138808299No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC10080.10527173265992844No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10070.10516729641721026No Hit
ATTTATTACTGTGCGCGAGCTACTAGTTATGATTACGATTGGGGGAGTCA10050.10495842393177389No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9870.1030785715628466No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA9760.10192977289294658No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAG9700.10130315543663748No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGGGGATAGCGGTGACTCCCCCAATCG9610.10036322925217384No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT9590.10015435676673748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)