FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA30570.31926159398948534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26850.2804113116983213No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT26300.27466731834882124No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTGTATAAAACACT25410.26537249274690294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21820.22787988161107525No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA21170.22109152583439334No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20230.21127451901888417No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19450.2031284920868659No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAATGAATTCTTGGCGTTGTCTCTGGA19330.2018752571742477No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17940.18735861943642027No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17720.18506102209662023No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG17180.17942146498983835No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16830.17576619649470193No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16830.17576619649470193No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16710.17451296158208374No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16330.17054438435879282No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16050.16762016956268366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15650.16344271985395636No Hit
ATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15590.16281610239764727No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15590.16281610239764727No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15050.1571765452908654No Hit
GTCCAGAGACAACGCCAAGAATTCATTGTCTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCATTTATTA14950.15613218286368355No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14720.15373014928116535No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCACGTCATCCAATAAGTAC14350.14986600830059257No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA13690.1429732162811925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13650.14255547131031976No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC13540.14140667264041976No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACTTATTGGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGG13180.13764696790256517No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12900.13472275310645604No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12880.13451388062101968No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12860.1343050081355833No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG12640.1320074107957833No Hit
CAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGA12630.1319029745530651No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12510.1306497396404469No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC12110.12647228993171958No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC11970.12501018253366503No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11860.12386138386376502No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11770.12292145767930138No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11740.12260814895114683No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11740.12260814895114683No Hit
GCATAAAGGAAGATGGAAGTGAGAAATTCTATGTGGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGACAACG11740.12260814895114683No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11730.12250371270842862No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCAC11720.12239927646571044No Hit
ATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCACCATCAGCAGCC11550.12062386033950133No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11540.12051942409678315No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAA11460.11968393415503768No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11250.11749077305795584No Hit
GTCTCTGGACATGGTGAATCGGCCCTTCACAGAGTCCACATAGAATTTCTCACTTCCATCTTCCTTTATGCTGGC11050.1154020482035922No Hit
GTCCAGTGTGACGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCC10860.1134177595919467No Hit
CCTTTAGTACTTATTGGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGG10760.11237339716476488No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10620.11091128976671032No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC10610.11080685352399214No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10550.11018023606768304No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTTGGAGGTGTCCAGTGTGACGTGCAGCTGGTGGAGT10450.1091358736405012No Hit
GGGCTGGAGTGGGTGGCCAGCATAAAGGAAGATGGAAGTGAGAAATTCTA10350.10809151121331938No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10290.10746489375701028No Hit
CTAGTAGCTCGCGCACAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAATGAATT10270.10725602127157391No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10210.10662940381526481No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10100.10548060514536481No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10080.10527173265992844No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10080.10527173265992844No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10070.10516729641721026No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC9820.10255639034925568No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT9740.10172090040751022No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9700.10130315543663748No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9590.10015435676673748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.056.8428461
TGGTATC2250.044.4678462
TCGTATG404.8906582E-543.28891840
GTATCAA14600.040.8940351
GGTATCA5900.039.7762261
CTCGTAT459.8082586E-538.46895239
TATACAC852.9704097E-936.5265663
TATCAAC16700.035.3272822
AGTAGCG400.002597111734.497315
TGTTAGA5300.032.8231670
ATCAACG17850.032.661323
TCAACGC17950.032.4793624
CAACGCA18350.031.9593645
AACGCAG18800.031.3778726
ATGATAC553.2581133E-431.3611933
CGATTAA800.004337921331.06477770
CTTATAC802.7537062E-630.1977631
GAGCAAA8350.029.76265770
ATGCCGT703.715849E-529.72590344
TACACAT1052.3319444E-829.5691225
Produced by FastQC (version 0.11.5)