FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957522
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26980.28176898285365765No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24870.25973293564012107No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTGTATAAAACACT23410.24448524420326634No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT23020.2404122307372572No Hit
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA22890.23905455958192082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18950.19790667995095673No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAATGAATTCTTGGCGTTGTCTCTGGA18800.196340136310184No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18310.19122276041699304No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17560.18339004221312932No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17380.18151018984420203No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17150.1791081562616838No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16040.1675157333199655No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15880.1658447534364746No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG15650.16344271985395636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15550.16239835742677453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15430.16114512251415633No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15320.15999632384425633No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15090.1575942902617381No Hit
ATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14840.15498338419378355No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14760.15414789425203806No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14620.1526857868539835No Hit
GTCCAGAGACAACGCCAAGAATTCATTGTCTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCATTTATTA14120.14746397471807435No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13980.1460018673200198No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13970.1458974310773016No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCCA13210.13796027663071972No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACTTATTGGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGG13180.13764696790256517No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC13040.1361848605045106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13010.13587155177635604No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12790.13357395443655604No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12680.132425155766656No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCACGTCATCCAATAAGTAC12270.12814326981521051No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12210.12751665235890142No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12060.12595010871812867No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG12020.12553236374725593No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11950.12480131004822867No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11880.12407025634920137No Hit
CAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGA11790.12313033016473772No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11620.12135491403852863No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11430.11937062542688313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11240.11738633681523766No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC11210.11707302808708311No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11120.11613310190261947No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCAC11080.11571535693174675No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11070.11561092068902855No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA11000.11487986699000127No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTTGGAGGTGTCCAGTGTGACGTGCAGCTGGTGGAGT10970.11456655826184672No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10940.11425324953369217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10820.11300001462107398No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10790.11268670589291943No Hit
GGGCTGGAGTGGGTGGCCAGCATAAAGGAAGATGGAAGTGAGAAATTCTA10730.11206008843661033No Hit
GTCTCTGGACATGGTGAATCGGCCCTTCACAGAGTCCACATAGAATTTCTCACTTCCATCTTCCTTTATGCTGGC10690.11164234346573759No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10550.11018023606768304No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC10510.1097624910968103No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10420.10882256491234665No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10350.10809151121331938No Hit
GTCCAGTGTGACGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCC10300.10756932999972846No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10050.10495842393177389No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10010.10454067896090116No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA10000.10443624271818297No Hit
CCTTTAGTACTTATTGGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGG9960.10401849774731024No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9950.10391406150459206No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGGGGATAGCGGTGACTCCCCCAATCG9920.10360075277643752No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA9860.10297413532012842No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9850.10286969907741023No Hit
ATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCACCATCAGCAGCC9800.10234751786381932No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC9800.10234751786381932No Hit
GCATAAAGGAAGATGGAAGTGAGAAATTCTATGTGGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGACAACG9790.10224308162110113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9760.10192977289294658No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9750.1018253366502284No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9650.10078097422304658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.054.4993021
TGGTATC2150.046.5275422
GTATCAA16850.045.2705351
TATCAAC18750.040.4736182
GGTATCA6000.038.5431181
ATCAACG20300.037.178343
TCAACGC20400.037.141744
CAACGCA20650.036.6945
AACGCAG21000.035.9184236
CGCAGAG23300.032.5206458
AGAGTAC23500.031.80584711
CAGAGTA23600.031.66942210
GCAGAGT24050.031.3632779
ACGACCC450.00424369431.20697463
ACGCAGA25300.029.9498447
CTGTATA6750.027.98497860
GTATACA500.00771655227.6129781
AGCGCTG3000.027.5538585
AGTACGG22200.026.69340713
TGTATAA7100.026.6365261
Produced by FastQC (version 0.11.5)