FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311783
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.038.9494971
TATCAAC43650.034.224112
GGTATCA17150.033.249651
TCAACGC46100.032.405264
ATCAACG47200.031.722813
CAACGCA47150.031.6836175
AACGCAG48250.031.1036476
ACGCAGA52300.028.562637
CGCAGAG52850.028.2653858
CTCGTAT1352.9467628E-1028.19961739
AGAGTAC53250.027.73061411
TCGTATG1404.456524E-1027.22072240
CAGAGTA54650.027.14589910
GCAGAGT57200.026.1758699
GAGTACG42400.025.51263612
AGTACGG42350.025.29949213
TAACGGC2050.025.27677236
TTAACGG2050.025.25352935
TCTCGTA1653.0522642E-923.05912838
AACGGCC2500.022.12147337
Produced by FastQC (version 0.11.5)