FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311783
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18130.13820883484539745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43950.037.8187331
GGTATCA20050.033.91341
TATCAAC51100.032.1763042
TCAACGC52400.031.079244
CAACGCA52600.030.9610675
AACGCAG53250.030.8406836
ATCAACG53300.030.6269473
TGGTATC9050.029.964022
ACGCAGA56750.028.8781997
TTGTACG605.6243286E-428.571094
CGCAGAG57200.028.5311328
GTGGTAT9750.028.5299431
AGAGTAC57850.027.56283611
CAGAGTA59150.026.95397610
GCAGAGT61650.026.5273199
GAGTACG47550.024.30450612
AGTACGG47250.024.16942813
TACGGGC11800.023.54290815
TTAACGG1803.43789E-1022.99978335
ACGGGTA2900.022.47221216
Produced by FastQC (version 0.11.5)