FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386617
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3980.10294425749514378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG306.283057E-446.03436725
CCTATAC306.315211E-445.9867173
GTATCAA9600.043.516861
GGTATCA3600.043.1572151
GTGTATA856.548362E-1140.618561
CAACGCA11850.035.4995425
ATCAACG11750.035.2238663
AACGCAG11950.035.2024776
TCAACGC11800.035.065544
ATATTAC400.002470206234.8462153
TATCAAC12050.034.369162
ACGCAGA12400.034.203047
GTAGTTT1459.901123E-833.1922970
CGCAGAG12800.033.1341978
AGAGTAC12550.032.97852311
CAGAGTA12950.031.9516110
TTAGTAC450.004625465330.657813
GCAGAGT14200.029.6207879
CGAAACA500.00768595427.63135329
GCGATAG500.00771516327.60989224
Produced by FastQC (version 0.11.5)