FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051468
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG40850.38850445282214957No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40320.3834638809740287No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG37210.35388618578977205No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32580.3098525109656214No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC31660.30110283907831714No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC31650.30100773394910735No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATG30220.2874077004721019No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAACCACTCAGG26240.2495558590465901No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC25680.24422997181083972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25370.24128171280533503No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24350.23158098962593252No Hit
GTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA23500.2234970536430971No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23210.22073900489601206No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22220.21132359710423904No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21280.20238371495851512No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21200.20162287392483652No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19890.189164101998349No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19190.18250674295366098No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18940.18012911472341525No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG18520.17613469929660247No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18110.17223538899899948No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16950.16120319401065938No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16890.1606325632354004No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16870.16044235297698076No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT16790.15968151194330213No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16730.15911088116804314No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC16690.15873046065120383No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16200.15407030931992224No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC16190.15397520419071242No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15790.15017099902231926No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15760.1498856836346898No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15640.14874442208417185No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15590.1482688964381227No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG15470.14712763488760475No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA15390.14636679385392615No Hit
GTGTATTACTGTGCTAGAGAAGTCGCCTCTAGCAGTGGCTGGGTCTACTGGGGCCAGGGAACCCTGGTCACCGT15220.14475000665735904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15210.14465490152814922No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15180.14436958614051973No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15010.14275279894395265No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14720.1399947501968676No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14660.13942411942160865No Hit
TATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT14530.1381877527418809No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG14460.13752201683741208No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13990.13305207576455014No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG13750.13076955266351423No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13520.1285821346916882No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13190.12544366542776383No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13180.125348560298554No Hit
CTCTAGCACAGTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTTGAAT13110.12468282439408523No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC13100.1245877192648754No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG13050.12411219361882625No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12780.12154435513016089No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12590.11973735767517414No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12480.11869120125386602No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12450.11840588586623654No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12440.11831078073702671No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAATATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC12390.11783525509097756No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12370.1176450448325579No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGTTTTCATTTGGTGAT12160.11564783711915151No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12060.11469678582705321No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11900.11317510375969597No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGC11900.11317510375969597No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11700.1112730011754994No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11670.11098768578786991No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11660.11089258065866009No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11620.11051216014182078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11520.10956110884972248No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG11470.10908558320367333No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11410.10851495242841437No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAATATGTATA11230.10680306010263745No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11230.10680306010263745No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG11220.10670795497342762No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11200.10651774471500797No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11170.10623242932737849No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11130.10585200881053917No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11110.10566179855211952No Hit
GGGCTGGAGTGGGTTGGCCGCACTAGAAACAAAGGTAACAGTTACACCAC11100.10556669342290968No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC10990.10452053700160156No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10990.10452053700160156No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10980.10442543187239174No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT10940.10404501135555241No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10880.10347438058029347No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAATAT10880.10347438058029347No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACC10820.10290374980503449No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10790.10261843441740501No Hit
GTGCTAGAGAAGTCGCCTCTAGCAGTGGCTGGGTCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT10750.10223801390056568No Hit
CAGTTACACCACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCAAAGAACTCA10640.10119185747925756No Hit
GTATACATATTTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA10630.10109675235004774No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10620.1010016472208379No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10560.10043101644557895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.044.725081
GTATCAA22200.043.2529951
GTGGTAT2350.041.1542361
TGGTATC2400.040.271912
TATCAAC25600.037.3504032
TAACAGT16850.036.48489470
ATCAACG27250.035.0637783
TCAACGC27350.035.0466884
CAACGCA27650.034.5417375
GTGTATA601.3317844E-534.5401571
AACGCAG28100.033.9885756
TCTTGAG9450.032.87729370
ACGCAGA29950.031.8891147
AGAGTAC29700.031.8168311
CGCAGAG30350.031.5824348
CAGAGTA30250.031.4618710
CCTATAC553.260081E-431.3583153
GCAGAGT31150.030.771339
GGATAAT900.005698150529.37971170
GTAGTTT1354.0219606E-529.3797170
Produced by FastQC (version 0.11.5)