FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559691
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32450.041.03441
GGTATCA13300.038.127751
ATCAACG38250.034.5394173
TATCAAC38550.034.3592
TCAACGC38700.034.2269254
CAACGCA39100.033.7003365
AACGCAG40400.032.70136
ACGCAGA42950.030.674557
CGCAGAG44850.029.4519718
AGAGTAC45250.028.80868111
CAGAGTA47700.027.47358910
GCAGAGT48650.027.2215259
AGTACGG37050.023.17721713
GAGTACG37100.023.05302612
GAGTACT20150.022.07825912
CGATAAG1303.318567E-521.91037669
GTGGTAT6650.021.7872871
AACGTCG800.002901040721.56102826
AGTACTT20700.021.15843613
CGAACTA2301.4551915E-1121.0086729
Produced by FastQC (version 0.11.5)