FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356695
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28550.040.790141
GGTATCA12400.037.0647741
ATCAACG32850.035.4416473
TATCAAC33200.034.9639552
CAACGCA33550.034.7021755
TCAACGC33550.034.59924
AACGCAG35700.032.612276
CGAATTA652.3023145E-531.885515
GTGGTAT4900.031.0304471
ACGCAGA37600.030.8678787
AGAGTAC37950.030.21910711
CGCAGAG39050.029.8101528
AACGTTA1004.4654778E-429.71795870
CAGAGTA39550.029.17126810
TATATCG605.379029E-428.7897645
GCAGAGT43650.026.7477829
TGGTATC5950.026.1285272
GAGTACG32350.024.87923212
AGTACGG32850.024.395413
GTGTATA2900.022.6405531
Produced by FastQC (version 0.11.5)