FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356695
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.038.3850061
GGTATCA12500.035.388681
GTGGTAT5400.033.9192661
TATCAAC31200.033.310062
ATCAACG30900.033.2675443
CAACGCA31750.032.6883935
TCAACGC31800.032.5285644
CCGATAA750.003479990932.4969170
AACGCAG33000.031.4501936
TGGTATC5950.029.5948892
ACGCAGA35350.029.3594467
CGCAGAG36250.029.0109948
AGAGTAC36400.028.42305211
CAGAGTA36950.027.99584810
GCAGAGT39200.026.475939
TATACTG2001.8189894E-1224.1361985
GTATAAG5300.022.8221511
AGTACGG32950.022.29497713
GAGTACG33150.022.15883412
TACGGGG15550.021.2931415
Produced by FastQC (version 0.11.5)