FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629720
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC17400.10676680656799942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48800.041.5845681
GGTATCA21950.037.0436861
GTGGTAT6200.035.0094761
ATCAACG58950.034.5945783
TATCAAC60100.034.161892
TCAACGC60650.033.7963334
AACGCAG61150.033.5763326
CAACGCA61400.033.4396175
ACGCAGA64850.031.5514957
GTAGTTT8150.030.60412670
CGCAGAG68450.029.841788
AGAGTAC68350.029.6829411
CAGAGTA68600.029.5756710
GCAGAGT72350.028.2807779
TGGTATC7450.027.7437462
AGTACGG56400.026.26149713
GAGTACG55850.026.21174212
GTACGGG62300.023.33213814
TCGGTAC1052.3080458E-522.9656543
TACGGGG24900.022.41020215
Produced by FastQC (version 0.11.5)