FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629720
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49250.040.579781
GGTATCA24350.035.0946271
GTGGTAT8000.034.8885571
TATCAAC59050.033.758742
ATCAACG59000.033.591653
TGGTATC8050.033.360022
TCAACGC60700.032.7471354
ACGGGTA2950.032.65553716
AACGCAG61300.032.4817126
CAACGCA61600.032.2686925
ACGCAGA63300.031.2924547
CAGAGTA65700.030.14935110
CGCAGAG66850.029.8879138
AGAGTAC66700.029.80319411
CGATTGT2751.8189894E-1229.16534870
GTAGTTT8100.028.1820970
GCAGAGT70750.028.1423039
AGTACGG56900.025.99782413
GAGTACG57300.025.81158312
TTATACG1759.094947E-1225.65957335
Produced by FastQC (version 0.11.5)