FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961562
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.041.5196721
GGTATCA10850.037.4220661
ATCAACG33500.033.6797563
TCAACGC33700.033.581954
CAACGCA34800.032.61935
TATCAAC35550.031.7376082
AACGCAG36050.031.5836756
TCGTATG1001.4069883E-831.14495540
ACGCAGA38400.029.7373057
AGAGTAC37550.029.3112811
CGCAGAG38800.029.1647958
TGGTATC4350.028.4677542
CGTATGC1103.529749E-828.33434941
CAGAGTA39000.028.30969410
GATCGTT500.00785024327.51596511
GCAGAGT40900.027.4991449
GTGGTAT4850.026.9598391
AGTACGG34350.025.73363713
GAGTACG34400.025.69623212
ACGGGTA2200.023.45110516
Produced by FastQC (version 0.11.5)