FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106621
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.039.9033471
GTCGTAC350.001351888839.40425530
GGTATCA10650.038.189671
TCGTACG400.002598958634.49277531
GTGGTAT4600.034.4677961
TCAACGC31450.034.246054
ATCAACG31850.033.825133
TATCAAC31850.033.7460442
CAACGCA32450.033.1892055
TGGTATC4500.032.9180152
AACGCAG33450.032.3013276
GTACGCT450.004618129730.6727533
ACGCAGA35350.030.3705047
CGCAGAG36150.029.6032168
AGAGTAC35000.029.40408911
CAGAGTA36250.028.57359910
GCAGAGT39150.027.4226659
TTAGCGA600.00817787527.28623469
GTATAAC1155.6899808E-826.9747961
TTAACGG1902.5465852E-1123.62814535
Produced by FastQC (version 0.11.5)