FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413260
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT103150.7298727764176444No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAACCTCGTA93110.6588313544570709No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT84120.5952195632792268No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68320.4834213095962526No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66500.4705432829061885No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC63150.4468392227898617No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT58470.4137242970154112No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56920.4027567468123346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55310.39136464627881634No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54290.3841472906613079No Hit
GTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAACCTCGTACA53650.3796187538032634No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG49320.34898037162305595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48910.34607927769837116No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46350.32796513026619306No Hit
GTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG44730.31650227134426784No Hit
GTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA44010.31140766737896775No Hit
GTCTGGGACCCCAGAGTCCCGCTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA43760.30963870766879414No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA42230.29881267424253144No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41330.2924444192859063No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG41190.2914538018482091No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39830.2818306610248645No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGAC39150.2770190906131922No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT38440.27199524503629907No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38030.2690941511116143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37800.2674667081782546No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA36640.2592587351230488No Hit
GAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA36330.2570652250824335No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCC35900.2540226143809349No Hit
GTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACCC35380.25034317818377366No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC33340.23590846694875678No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGA32720.23152144686752613No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACA32370.22904490327328303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32300.22854959455443444No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31830.225223950299308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30960.21906797050790372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29580.2093033129077452No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29240.20689752770190906No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29110.20597766865261877No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28880.204350225719259No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28370.2007415479105048No Hit
CAGTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG28260.1999632056380284No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG28210.19960941369599366No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27810.19677907815971582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27780.196566802994495No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27360.19359495068140328No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27000.19104764869875324No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGGCT26810.18970323931902128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26710.1889956554349518No Hit
TTCCATCACTGTGTACGAGGTTTTGACTAGACCTGCAGGAGATGGAGGCC26350.18644835345230176No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC25870.18305195080876838No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA25550.18078768237974613No Hit
AGTCAAAACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG25540.18071692399133915No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC24870.1759761119680738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24840.17576383680285296No Hit
CAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAAT24580.17392411870427238No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATT24450.17300425965498212No Hit
GTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGC24350.17229667577091265No Hit
TTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGA24040.17010316573029732No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT23940.16939558184622786No Hit
GGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGACTCTGG23920.16925406506941398No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT23790.16833420602012367No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23630.16720207180561256No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23560.16670676308676394No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22750.16097533362580135No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT22530.15941864908084852No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22400.15849879003155826No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTC22210.15715438065182627No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22090.1563052799909429No Hit
GTCCAAGGGTGACGGGCAGGGAGTGTGGAGACTGAGTCAGCACAACATCC21550.15248432701696787No Hit
GATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGG20990.1485218572661789No Hit
GGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20880.1477435149937025No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20730.1466821391675983No Hit
GTTTTCAAGCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC20710.14654062239078444No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20470.14484242106901773No Hit
GGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGA20170.14271966941680936No Hit
GCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCC20050.14187056875592602No Hit
CCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG20020.14165829359070517No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20000.1415167768138913No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19990.14144601842548432No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19720.13953554193849682No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19590.1386156828892065No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19340.13684672317903288No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGCCAGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGC19240.1361391392949634No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT19210.1359268641297426No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18940.13401638764275503No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18890.1336625957007203No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCACTGTGT18810.13309652859346477No Hit
AGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACC18510.1309737769412564No Hit
ACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGG18430.13040770983400082No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18310.12955860917311748No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTG17760.12566689781073548No Hit
GCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGACTCTGGGGTCCCAGACAGATT17720.12538386425710768No Hit
CAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17640.12481779714985212No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17400.12311959582808542No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17250.12205822000198123No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17240.12198746161357428No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17210.12177518644835345No Hit
GTGTACGAGGTTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGTGTGGAGA17150.12135063611791178No Hit
GGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGA17150.12135063611791178No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16940.11986470996136593No Hit
ACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGG16930.11979395157295898No Hit
GTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGC16740.118449542193227No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16530.11696361603668115No Hit
GTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGC16510.11682209925986725No Hit
GGAAATCAGCAGGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTT16500.11675134087146032No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16460.11646830731783253No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG16420.11618527376420473No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16300.1153361731033214No Hit
CCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGC16130.11413328050040332No Hit
CCCCAGAGTCCCGCTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGG16070.11370873016996164No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15960.11293038789748525No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGG15920.11264735434385745No Hit
GGTCTAGTCAAAACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC15800.11179825368297412No Hit
TCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACC15520.10981701880757963No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGCCAGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCT15470.10946322686554491No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15430.10918019331191713No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15150.10719895843652265No Hit
GGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGG15050.1064913745524532No Hit
GGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTC15000.10613758261041846No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14900.105429998726349No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14770.10451013967705872No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG14560.10302421352051286No Hit
CCAGAGTCCCGCTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCC14550.10295345513210591No Hit
CTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGC14440.1021751128596295No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA14410.10196283769440867No Hit
CTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT14410.10196283769440867No Hit
GATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGAAATCAGCAGG14380.10175056252918784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14350.101538287363967No Hit
GACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAACCT14310.10125525381033923No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14270.10097222025671143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC200.007287374751.8788440
AATTGCG200.00739248751.69127731
GGTATCA12050.047.4058231
TCGTATG551.44164E-744.0184140
GTATCAA31900.042.8262981
GTGGTAT3100.041.072461
CGTATGC702.1289452E-839.55487441
TGGTATC3350.037.995252
TATCAAC36550.037.6482662
ATCAACG37700.036.4085963
TCAACGC38000.036.121164
CAACGCA39000.035.2831845
AACGCAG39900.034.4873246
ACGCAGA42300.032.283197
AGAGTAC42000.032.2680911
CGCAGAG42600.032.0558438
GCAGAGT43150.031.5675339
CAGAGTA43400.031.30643810
AATGATT1105.527808E-428.65389870
AGTATCG500.006593696328.51782866
Produced by FastQC (version 0.11.5)