FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413260
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT104470.7392128836873612No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAACCTCGTA75890.5369854096203105No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT75280.5326691479274868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT62320.44096627655208526No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC59930.4240550217228253No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58960.4171914580473515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55300.3912938878904094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55250.3909400959483747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53630.3794772370264495No Hit
GTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAACCTCGTACA53360.3775667605394619No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51290.3629197741392242No Hit
GTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG50490.3572591030666686No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG49230.3483435461273934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45860.3244979692342527No Hit
GTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA44050.3116907009325956No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA41900.29647764742510224No Hit
GTCTGGGACCCCAGAGTCCCGCTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA41720.29520399643377726No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40590.2872082985437924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40460.28628843949450206No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40420.2860054059408743No Hit
GTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACCC40090.28367037912344506No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA39500.2794956342074353No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG37430.2648486478071975No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGAC36140.25572081570270155No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA34650.24517781583006665No Hit
GAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA34640.24510705744165973No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34530.24432871516918328No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT34170.2417814131865333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34030.24079079574883602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33900.23987093669954573No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32590.23060158781823586No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31640.22387954091957601No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCC31530.22310119864709962No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC31470.22267664831665795No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACA29890.21149682294836053No Hit
CAGTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG29130.20611918542943267No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT28010.19819424592785476No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27980.19798197076263394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27950.1977696955974131No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27920.19755742043219227No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGA27580.1951516352263561No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27360.19359495068140328No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG27300.1931704003509616No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26790.18956172254220738No Hit
GGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGA26750.1892786889885796No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26510.1875804876668129No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26230.18559925279141842No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25600.18114147432178085No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA25320.17916023944638637No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25230.17852341395072385No Hit
GCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCC25100.17760355490143356No Hit
GTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGC24970.17668369585214327No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGGCT24710.1748439777535627No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC24130.17073999122595984No Hit
TTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGA23870.16890027312737926No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23820.16854648118534452No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23540.16656524630995004No Hit
TTCCATCACTGTGTACGAGGTTTTGACTAGACCTGCAGGAGATGGAGGCC23490.16621145436791532No Hit
AGTCAAAACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG23300.16486704498818336No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATT23130.16366415238526527No Hit
GGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGACTCTGG22280.1576496893706749No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21590.15276736057059564No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21380.1512814344140498No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT20990.1485218572661789No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20810.14724820627485388No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20640.1460453136719358No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTC20540.14533772978786635No Hit
CAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAAT20480.14491317945742468No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20410.14441787073857607No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20010.14158753520229825No Hit
GTCCAAGGGTGACGGGCAGGGAGTGTGGAGACTGAGTCAGCACAACATCC19930.14102146809504265No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19880.14066767615300793No Hit
GATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGG19840.14038464259938016No Hit
GTTTTCAAGCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC19770.13988933388053154No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19760.1398185754921246No Hit
GGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19720.13953554193849682No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19630.1388987164428343No Hit
GTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGC19570.13847416611239263No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19560.13840340772398568No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT19500.137978857393544No Hit
CCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG19400.13727127350947455No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCACTGTGT19050.13479472991523145No Hit
AGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCTCCACC19000.13444093797319673No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18720.13245970309780225No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18400.13019543466878No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18390.13012467628037303No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18230.1289925420658619No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGCCAGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGC18060.12778964946294383No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18030.12757737429772298No Hit
ACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGG17660.12495931392666601No Hit
GGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGA17490.12375642132374794No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17220.1218459448367604No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16810.11894485091207563No Hit
GTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGCTT16710.11823726702800616No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16580.11731740797871588No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16500.11675134087146032No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16400.11604375698739085No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16300.1153361731033214No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTG16220.11477010599606582No Hit
ACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGCTTGAAAACC16220.11477010599606582No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA16170.1144163140540311No Hit
TCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACC16100.11392100533518248No Hit
GGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTC16070.11370873016996164No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16030.11342569661633387No Hit
CAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15640.11066611946846298No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15640.11066611946846298No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15610.11045384430324215No Hit
ACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGAAATCAGCAGGGTGGAGG15560.11010005236120743No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15550.11002929397280048No Hit
GGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGG15550.11002929397280048No Hit
GTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGC15550.11002929397280048No Hit
GGTCTAGTCAAAACCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC15480.10953398525395186No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15390.10889715975828936No Hit
GTGGGGATGTTGTGCTGACTCAGTCTCCACACTCCCTGCCCGTCACCCTT15380.1088264013698824No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15240.10783578393218517No Hit
GTGTACGAGGTTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGTGTGGAGA15200.10755275037855738No Hit
GCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTTCAAGCGGGACTCTGGGGTCCCAGACAGATT15000.10613758261041846No Hit
GGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTTCGGCGGAGGGAC14980.10599606583360457No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGCCAGTGTGTTCCTTGCATGCAGTAATAAACTGCAACATCCTCAGCCT14790.1046516564538726No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14750.10436862290024483No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14730.10422710612343093No Hit
CCCCAGAGTCCCGCTTGAAAACCTTGTAAATTAGGCGCCTTGGAGATTGG14730.10422710612343093No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14530.10281193835529202No Hit
CTCCACACTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGC14520.10274117996688506No Hit
CCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGC14500.10259966319007119No Hit
GGAAATCAGCAGGGTGGAGGCTGAGGATGTTGCAGTTTATTACTGCATGCAAGGAACACACTGGCCTCTCACTTT14430.10210435447122256No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG14370.1016798041407809No Hit
GCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGT14310.10125525381033923No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGG14310.10125525381033923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)