FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974545
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT35490.36416994597478825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30620.3141979077415614No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT22670.23262137715549308No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATT19740.20255606462503015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18210.18685643043676795No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18120.18593292254334073No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18110.18583031055518215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17400.17854485939592324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16830.17269597607088438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16380.1680784366037484No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT15500.1590485816457937No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG15020.15412320621418202No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14630.1501213386759975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14360.14735081499571595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14200.14570902318517873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14070.14437506733911723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14030.14396461938648292No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13790.1415019316706771No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12320.12641796941136632No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCT12070.12385266970740191No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11910.12221087789686469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11740.12046647409816888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11420.11718289047709443No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10750.11030788727046982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10690.10969221534151835No Hit
GTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATTCA10530.10805042353098113No Hit
CATCTATGGTGCATTCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10170.10435639195727237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10150.10415116798095521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.043.4803661
GTATCAA22700.042.9844061
ATCAACG28750.033.8971863
TCAACGC28850.033.891684
TATCAAC28950.033.8075682
GTAGTTT3400.033.6641770
CAACGCA29200.033.4854435
AACGCAG29800.032.811246
CGCAGAG31800.030.8555248
AGAGTAC32250.030.3263811
GCAGAGT33800.029.0297539
ACGCAGA34300.028.606587
ACTCGAC1502.910383E-1128.03906457
CAGAGTA35400.027.7205210
AGTACGG27450.027.63121413
GAGTACG27700.027.38042812
GTCGGTT809.246383E-426.2395869
TACGGGA10200.025.24063715
GTACGGG31450.023.79075214
TGTTAGA7700.023.12286470
Produced by FastQC (version 0.11.5)