FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734224
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9890.13470003704591516No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT8090.1101843579071237No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA7450.10146767199110898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2100.042.734611
GTATCAA14200.041.07941
GGTATCA5800.038.682191
TCAACGC17500.033.5256654
ATCAACG17700.033.3418273
CAACGCA17750.033.0534745
AACGCAG18700.031.372156
TGGTATC2650.029.9535642
ACGCAGA19700.029.7776267
TATCAAC19900.029.3089432
AGAGTAC19700.029.2541311
CAGAGTA20100.028.50027310
CGCAGAG21050.027.7039688
GTGTATA1800.026.8461021
GCAGAGT22100.026.3877169
CGACTGT750.001732223123.55981866
GAGTACG19850.023.29599412
GAACCGA750.00186862723.25489855
AGTAACG750.001892701123.20378151
AGTACGG20100.023.17793313
Produced by FastQC (version 0.11.5)