FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279484
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14790.11559347361905269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.040.266211
TCGCTAA650.00180925137.1333570
TATCAAC39300.034.3397832
TCAACGC39450.034.2938044
CAACGCA40200.033.9135485
ATCAACG39950.033.865923
AACGCAG40950.033.292426
CTAGCGC851.228218E-732.41111828
TATCGGC450.004676135330.59494810
AGAGTAC45550.029.99882911
CGCAGAG45900.029.7724118
ACGCAGA46050.029.6006837
GGTATCA17900.029.4313641
CAGAGTA47800.028.80277410
TCGTATG854.3215605E-628.50126540
GCAGAGT48300.028.2908239
AGTACGG37050.026.38143313
GAGTACG37650.025.869612
GTACGGG42650.022.91751714
TTAACGG1554.076901E-822.26343735
Produced by FastQC (version 0.11.5)