FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738137
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA15030.20362073707184436No Hit
GTGTATTACTGTGCGAGACTCACCCTTCTAATGATTTTTGGAGTGGCTCCAAACGACTACTGGGGCCAGGGAATC11780.15959097023994193No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10490.14211453971281754No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9630.13046358602806796No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9540.12924430017733837No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9000.1219285850729607No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8990.12179310886732408No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8770.11881263234331838No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8720.11813525131513526No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8200.11109048862203087No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7750.10499405936838282No Hit
GTATTACTGTCAGCAGTATGGTACCTCATTGACGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7710.10445215454583634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.041.0157171
GCGGCTA808.390882E-539.49500370
TCGTATG1009.094947E-1238.08839840
CGTATGC1100.037.7941841
GTATCAA15600.037.1554151
CTCGTAT952.1100277E-1036.43831639
AATCTCG952.1464075E-1036.38389636
TACGGAT4950.035.74497270
CTTATAC1300.034.5014571
TATCAAC17850.032.660822
TCAACGC18050.032.1078074
ATCAACG18150.031.9309063
TTATACA1207.8216544E-1131.6220512
TCGTCCG553.1617878E-431.52088744
CAACGCA18500.031.5132755
AACGCAG19300.030.2090746
TTCGGGG4750.029.26787870
TGTTAGA2202.6993803E-928.72363970
GTGGTAT4050.028.1122991
AGTTTCG900.00709866128.08533770
Produced by FastQC (version 0.11.5)