FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382088
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46190.3342044790201492No Hit
GTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA37440.27089447271085487No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG36110.26127135175184213No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32370.23421084619792665No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32090.2321849259960292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31380.22704777119836073No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30650.22176590781484246No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29660.21460283281527662No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29360.21243220402752935No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28070.20309850024021625No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG27310.19759957397792327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27070.19586307094772545No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26870.1944159850892273No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26540.19202829342270536No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26440.1913047504934563No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTT26060.18855528736230978No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCAGGCGAGTCAGGACATTAGCAAGTACTTAAATTGGTATCAGCAGAAA25960.1878317444330607No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG25930.18761468155428598No Hit
GTATGATAATCTCCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT25730.18616759569578784No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25600.185226989887764No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25400.18377990402926586No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG25360.18349048685756625No Hit
GTGTATTACTGTGTGAGAGGAGGCCCGGCTAGATATGGTTCGGGGAGTTATAGGGACTACTGGGGCCAGGGAACC24730.17893216640329704No Hit
CAGTAATACACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA24310.1758932861004509No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT24280.1756762232216762No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23780.17205850857543079No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22340.16163949039424408No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22200.16062653029329535No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG21280.15396993534420386No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21110.1527399123644804No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGCTAC20920.15136518079890718No Hit
GTACTATGTGGGCTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACG20910.15129282650598225No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20200.1461556717083138No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19930.14420210579934128No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC19920.14412975150641638No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC19740.14282737423376804No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTC19670.1423208941832937No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT19630.14203147701159405No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19470.14087380832479554No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19200.13892024241582301No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCAGGCGAGTCAGG19130.13841376236534866No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18960.13718373938562523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18880.13660490504222597No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18700.13530252776957763No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC18570.13436192196155383No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT18170.1314677502445575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17830.12900770428511066No Hit
AGTCAGGACATTAGCAAGTACTTAAATTGGTATCAGCAGAAACCAGGGAA17750.12842886994171138No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17160.12415996665914182No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16990.12292994367941838No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16920.12242346362894402No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16670.12061460630582134No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT16510.11945693761902282No Hit
GTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA16460.11909516615439827No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16380.118516331810999No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA16310.11800985176052466No Hit
GCCTACAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCTCTCACCTTCGGCCAAGGGA16200.11721395453835068No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16130.11670747448787631No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT15840.114609199993054No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15790.11424742852842945No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15700.11359623989210528No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC15630.11308975984163092No Hit
CTCCTGTGCGGCCTCTGGATTCACCTTTAGTAGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15530.11236621691238183No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT15510.11222150832653204No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15460.11185973686190749No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15350.1110638396397335No Hit
GGACATTAGCAAGTACTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATC15150.10961675378123535No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA15060.10896556514491118No Hit
CCCTATAACTCCCCGAACCATATCTAGCCGGGCCTCCTCTCACACAGTAA14750.10672258206423904No Hit
ACATAAAGCAATATGGAAGTGAGAAGTACTATGTGGGCTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACG14710.1064331648925394No Hit
GTTATAGGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCC14710.1064331648925394No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14700.1063608105996145No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA14630.10585433054914013No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAATATGGAAGTGAGAAGTACTA14580.1054925590845156No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14320.10361134746846799No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14320.10361134746846799No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC14280.10332193029676837No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCGGC14210.102815450246294No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA14060.1017301358524204No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13950.1009342386302464No Hit
GTATTACTGTGTGAGAGGAGGCCCGGCTAGATATGGTTCGGGGAGTTATAGGGACTACTGGGGCCAGGGAACC13910.10064482145854678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19950.039.478691
GTGGTAT3150.037.285431
ACGGGTA400.002585892834.52885416
TATCAAC22900.034.3818052
ATCAACG24300.032.543073
TCAACGC24400.032.4096954
AACGCAG25350.031.330226
CAACGCA25300.031.256785
AGAGTAC26800.029.24433911
CGCAGAG27500.029.0021388
CAGAGTA27700.028.41880410
GCAGAGT28250.028.2321689
TCTGGAG17700.027.59129170
GCTGATT1156.917583E-427.57571470
AGCGCTG6050.027.3977665
CAGCGCT5850.027.1538414
ACGGGAC1607.8216544E-1125.89664316
ACGCAGA31150.025.4929737
TGTTAGA6050.025.15998870
CGCACAG10600.025.13031870
Produced by FastQC (version 0.11.5)