FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854108
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11790.1380387491979937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.042.609421
GTATCAA25050.039.429911
TATCAAC29950.032.848622
TCAACGC30500.032.111494
ATCAACG30700.031.7996673
TTAACGG1850.031.6973635
CAACGCA31200.031.499345
AACGCAG31700.030.8941046
GTGGTAT4200.030.3182431
AGAGTAC33650.029.0033911
CGCAGAG34500.028.5859648
ACGCAGA34650.028.4622157
TAACGGC2100.027.94356736
CAGAGTA35450.027.72297710
GTATACG500.00782713327.5322421
TGGTATC4250.027.5193352
GCAGAGT36000.027.4903349
TTTAACG2350.024.94737434
AGTACGG28300.024.89915513
CAATCGT851.302218E-424.723755
Produced by FastQC (version 0.11.5)