FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64690.43116922242239925No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA58260.388312241433436No Hit
CTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT45550.3035980535065742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42030.2801366891082615No Hit
GTATAATCACTGGTTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40810.2720051934929372No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38540.25687527952016176No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT37300.24861048069802894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36350.24227857837462066No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT33840.22554902592014206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33420.22274965857716156No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC32750.21828400114907365No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32490.21655105946056194No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA32320.21541798220268885No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC31870.21241866004949547No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCAC31760.2116854924120482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31420.2094193378963021No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA30400.20262087434906378No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT29920.19942159738565748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28730.19149005658054613No Hit
GTGATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT28180.18782421839330976No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTA27680.18449163822309492No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26320.17542702016011047No Hit
GTAATACACGGCTGTGTCCGCGGCAGTCACGGAGCTCAGGTTCAGGGAGA26220.17476050412606753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25430.16949502745712802No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24230.16149683504861234No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT23890.15923068053286624No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23000.1532986878298838No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA22840.15223226217541502No Hit
GTCAGAGTGTTGCCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTG22830.15216561057201072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21310.14203456685455754No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGGTGGCA20960.13970176073540713No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAATTACCACTGGCACTGGATCCG20110.13403637444604186No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20020.1334365100154032No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG19530.13017058144859261No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19510.13003727824178404No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19470.12977067182816684No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT19290.12857094296688948No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG19050.12697130448518634No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18850.1256382724171004No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18800.1253050144000789No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18770.125105059589866No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA18560.12370537591837578No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18500.12330546629795No Hit
ACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTA18290.12190578262645975No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC18260.12170582781624685No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18160.1210393117822039No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17780.11850655085284059No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17580.11717351878475464No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17320.11544057709624292No Hit
GTTTATTACTGTCAGCAGTATAATCACTGGTTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17280.11517397068262572No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17060.11370763540773118No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17010.1133743773907097No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG16960.11304111937368823No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC16890.11257455814985813No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16880.11250790654645383No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16660.1110415712715593No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC16570.11044170684092061No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT16110.10737573308432294No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16100.10730908148091865No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16070.10710912667070577No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15790.10524288177538542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15700.10464301734474676No Hit
GCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT15480.10317668206985221No Hit
GTGTATTACTGTGCGAGAGGGGAGGATTACTTTGAAACTAGTGGTTACTC15470.10311003046644793No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC15270.10177699839836198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)